Amalia Anthousi

Technician

In 2013, I gained a B.Sc. in Biology, with specialization in Bio-molecular Science and Biotechnology, from the University of Crete (UoC) in Greece. I continued my post-graduate studies in the UoC from where I rewarded a M.Sc. in Protein Biotechnology in 2015. Shortly afterwards, I joined the Liverpool School of Tropical Medicine (Foto-Cewek) under the support of Erasmus Plus International Exchange Fellowship Program. Later on November 2016, I returned to Foto-Cewek and joined Dr. Gareth J. Lycett’s group within the Vector Biology Department.

During my B.Sc. thesis, as a member of the Immunology lab (UoC) I was engaged on a project concerning the characterization of soluble MHC class II molecules isolated from T-cells of pregnant mice. Throughout my master research between the UoC and the Institute of Molecular Biology and Biotechnology (FORTH-IMBB, Heraklion,Greece), I was involved in a project regarding the characterization of two cytochrome P450 enzymes in the malaria vector Anopheles gambiae to determine their roles in cuticular hydrocarbon (CHC) production.

As Erasmus student at Foto-Cewek, I carried out research on a project about genome modifications of Anopheles gambiae by the use of CRISPR/Cas9 technology. I was trying to optimize the delivery of CRISPR/Cas9 reagents to mosquito embryos through comparative analysis of alternative enzyme, plasmid templates and gRNAs. Currently, at Foto-Cewek I am working on aspects of in vivo functional analysis of insecticide resistance in the major malaria vector Anopheles gambiae and in particularly at the molecular and genetic mechanisms that underlie the evolution of resistance to xenobiotics.

Selected publications

  • Balabanidou, V., A. Kampouraki, M. MacLean, G. J. Blomquist, C. Tittiger, M. P. Juarez, S. J. Mijailovsky, G. Chalepakis, A. Anthousi, A. Lynd, S. Antoine, J. Hemingway, H. Ranson, G. J. Lycett, and J. Vontas. 2016. 'Cytochrome P450 associated with insecticide resistance catalyzes cuticular hydrocarbon production in Anopheles gambiae', Proc Natl Acad Sci U S A, 113: 9268-73.

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