Areas of interest
Cytoadhesion in malaria
Tadge Szestak graduated in Pure & Applied Biology from Oxford University (Keble College) in 1991. He spent the next four years as a Research in Medecine Technician at the CRC Institute for Cancer Studies in Birmingham involved in the study of early region genes in adenovirus, and then five years as a Research in Medecine Assistant at the The Babraham Institute, Cambridge working in the field of insulin-like growth factors. He then spent three years as a Research in Medecine Scientist with Ionix Pharmaceuticals on the Cambridge Science Park working within a number of drug discovery programmes in the field of pain relief, before joining the Liverpool School of Tropical Medicine in June 2005.
Research in Medecine
I am currently working in Professor Alister Craig's group within a project looking at the correlation between severity of disease and the binding profile of Plasmodium falciparum-infected erythrocytes from patient isolates. The work has involved the employment of binding assays - under both static and flow conditions - to protein and stimulated endothelial cell layers, the production and purification of ICAM-1 protein (which has been shown to be important in the binding of infected erythrocytes to endothelial cells), and also the molecular cloning of the DBLβ2 domain of the malarial protein PfEMP1 from each ICAM-1 binding isolate, and comparing the sequences of these domains. The DBLβ2 domain is thought to be responsible for the binding of infected erythrocytes to ICAM-1.
In addition I am associated with a project aiming to create a novel set of Plasmodium falciparum lines whereby monoclonal antibodies raised against DBLβ2 domains of PfEMP1 will be used to select parasite lines that express PfEMP1 proteins containing specific ICAM-1-binding DBL domains. The antibodies will also be tested for their ability to bind to infected erythrocytes and to inhibit ICAM-1 adhesion.
My role as Laboratory Manager encompasses the following roles:
- Management of all areas pertaining to Health and Safety in laboratories and good laboratory practice
- Chairman of the Laboratory User Group
- Human Tissue Authority (HTA) Licence Coordinator
- Laboratory Training Coordinator
- Laboratory Equipment Maintenance Coordinator
- Membership of:
- Governance Oversight Committee
- Health, Safety and Environment Management Committee
- Laboratory Audit Team
- Training Working Group
- Containment Level 3 User Group
- Genetically Modified Organisms (GMO) and Pathogens Group