Publications

2017

Slagboom J, Kool J, Harrison RA, Casewell NR. (2017). Haemotoxic snake venoms: their functional activity, impact on snakebite victims and pharmaceutical promise. British Journal of Haematology. In press.

Han H*, Baumann K*, Casewell NR*, Ali SA, Dobson J, Koludarov I, Debono J, Cutmore S, Rajapakse NW, Jackson TNW, Jones R, Hodgson WC, Fry BG, Kuruppu S. The cardiovascular and neurotoxic effects of the venoms of six cartilaginous and bony fish species. Toxins. In press. *these authors contributed equally. 

Pla D, Sanz L, Whiteley G, Wagstaff SC, Harrison RA, Casewell NR, Calvete JJ. 2017. What killed Karl Patterson Schmidt? Combined venom gland transcriptomic, venomic and antivenomic analysis of the South African green tree snake (the boomslang), Dispholidus typus. Biochimica et Biophysica Acta (BBA) – General Subjects. 1861: 814–823

2016

Whiteley, G, Logan, RE, Leung, K-Y, Newberry, F, Rowley, PD, Dunbar, JP, Wagstaff, SC, Casewell, NR, Harrison, RA. (2016) Stabilising the Integrity of Snake Venom mRNA Stored Under Tropical Field Conditions Expands Research in Medecine Horizons. PLOS Neglected Tropical Diseases. doi:10.1371/journal.pntd.0004615

 Harrison RA, and Gutiérrez J-M. (2016) Priority Actions and Progress to Substantially and Sustainably Reduce the Mortality, Morbidity and Socioeconomic Burden of Tropical Snakebite. Toxins 8, 351; doi:10.3390/toxins8120351.

 Simões BF, Sampaio FL, Douglas RH, Kodandaramaiah U, Casewell NR, Harrison RA, Hart NS, Partridge JC, Hunt DM, Gower DJ. (2016). Visual pigments, ocular filters and the evolution of snake vision. Molecular Biology and Evolution. 33(10), 2483-2495. doi: 10.1093/molbev/msw148

 Casewell NR. (2016). Venom evolution: gene loss shapes phenotypic adaptation. Current Biology. 26, R849-R851. doi: 10.1016/j.cub.2016.07.082.

2015

Gutiérrez J-M, Burnouf T, Harrison RA, Calvete JJ, Brown N, Warrell, DA & Williams DJ, (2015) A call for incorporating social research in the global struggle against snakebite. PLoS NTD DOI:10.1371/journal.pntd.0003960.

Ujvari B*, Casewell NR*, Sunagar K*, Arbuckle K, Wüster W, Lo N, O’Meally D, Beckmann C, King GF, Deplazes E, Madsen T. (2015) Widespread convergence in toxin resistance by predictable molecular evolution. Proc. Natl. Acad. Sci. USA. 112(38), 11911-11916.*these authors contributed equally.

Dunbar JP, Dunbar T. 2015. Ahaetulla prasina (Asian Vinesnake). Diet and feeding behavior. Herpetological Review. 46(2).

Jouiaei M*, Casewell NR*, Yanagihara AA, Nouwens A, Cribb BW, Whitehead D, Jackson TNW, Ali SA, Wagstaff SC, Koludarov I, Alewood P, Hansen J, Fry BG. 2015. Firing the sting: chemical discharge of cnidae reveals novel proteins and peptides from box jellyfish (Chironex fleckeri) venom. Toxins. 7, 936-950. doi: 10.3390/toxins7030936. *these authors contributed equally.

Junqueira-de-Azevado ILM, Val Bastos CM, Ho PL, Luna MS, Yamanouye N, Casewell NR. 2015. Venom-related transcripts from Bothrops jararaca tissues provide novel molecular insights into the production and evolution of snake venom. Molecular Biology and Evolution. 32(3), 754-766. doi: 10.1093/molbev/msu337.

Reyes-Velasco J, Card DC, Andrew AL, Shaney KJ, Adams RH, Schield DR, Casewell NR, Mackessy SP, Castoe TA. 2015. Expression of venom gene homologs in diverse python tissues suggests a new model for the evolution of snake venom. Molecular Biology and Evolution. 32(1), 173-183. doi: 10.1093/molbev/msu294.

Lecht S, Ciaverelli RA, Gerstenhaber J, Calvete JJ, Lazarovici P, Casewell NR, Harrison R, Lelkes PI, Marcinkiewicz C. 2015. Anti-angiogenic activities of snake venom CRISP isolated from Echis carinatus sochureki. Biochimica et Biophysica Acta (BBA) – General Subjects. 1850(6), 1169-1179. doi: 10.1016/j.bbagen.2015.02.002.

Ali SA, Jackson TNW, Casewell NR, Low DHW, Rossi S, Baumann K, Fathinia B, Visser J, Nouwens A, Hendrikx I, Jones A, Undheim E, Fry BG. 2015. Extreme venom variation in Middle Eastern vipers: a proteomics comparison of Eristicophis macmahonii, Pseudocerastes fieldi and Pseudocerastes persicus. Journal of Proteomics. 116, 106-113. doi: 10.1016/j.prot.2014.09.003.

Casewell NR, Sunagar K, Takacs Z, Calvete JJ, Jackson TNW, Fry BG. 2015. Snake venom metalloprotease enzymes. pp. 347-363. In: Fry BG (Editor), Venomous reptiles & their toxins: evolution, pathophysiology and biodiscovery. Oxford University Press, New York, USA. ISBN: 978-0199309399.

Fry BG, Sunagar K, Casewell NR, Kochva E, Roelants K, Scheib H, Wüster W, Vidal N, Young B, Burbrink F, Pyron RA, Vonk FJ, Jackson TNW. 2015. The origin and evolution of the Toxicofera reptile venom system. pp. 1-31. In: Fry BG (Editor), Venomous reptiles & their toxins: evolution, pathophysiology and biodiscovery. Oxford University Press, New York, USA. ISBN: 978-0199309399.

Fry BG, Undheim EAB, Jackson TNW, Georgieva D, Vetter I, Calvete JJ, Scheib H, Cribb BW, Yang DC, Daly NL, Manchadi MLR, Gutiérrez JM, Roelants K, Lomonte B, Nicholson GM, Dziemborowicz S, Lavergne V, Ragnarsson L, Rash LD, Mobli M, Hodgson WC, Casewell NR, Nouwens A, Wagstaff SC, Ali SA, Whitehead DL, Herzig V, Monagle P, Kurniawan ND, Reeks T, Sunagar K. 2015. Research in Medecine methods. pp. 153-214. In: Fry BG (Editor), Venomous reptiles & their toxins: evolution, pathophysiology and biodiscovery. Oxford University Press, New York, USA. ISBN: 978-0199309399.

Fry BG, Koludarov I, Jackson TNW, Holford M, Terrat Y, Casewell NR, Undheim EAB, Vetter I, Ali SA, Low DHW, Sunagar K. 2015. Seeing the woods for the trees: understanding venom evolution as a guide for biodiscovery. pp. 1-36. In: King GF (Editor), Venoms to drugs: venom as a source for the development of human therapeutics. Royal Society of Chemistry, Cambridge, UK. ISBN: 978-1849736633. 

2014

Gutiérrez J-M, Burnouf T, Harrison RA, Calvete JJ, Kuch U, Warrell DA & Williams DJ, (2014) A multicomponent strategy to improve the availability of antivenom for treating snakebite envenoming, Bull WHO, 92(7):526-32. doi: 10.2471/BLT.13.132431.

Casewell NR, Wagstaff SC, Wüster W, Cook DAN, Bolton FMS, King SI, Pla D, Sanz L, Calvete JJ, Harrison RA. 2014. Medically important differences in snake venom composition are dictated by distinct postgenomic mechanisms. Proc. Natl. Acad. Sci. USA. 111(25), 9205-9210. doi: 10.1073/pnas.1405484111. Cover article.

Archer J, Whiteley G, Casewell NR, Harrison RA, Wagstaff SC. 2014. VTBuilder – a tool for the assembly of variant transcriptomes. BMC Bioinformatics. 15, 389. doi: 10.1186/s12859-014-0389-8.

Baumann K*, Casewell NR*, Ali SA, Jackson TNW, Vetter I, Dobson JS, Cutmore SC, Nouwens A, Lavergne V, Fry BG. 2014. A ray of venom: combined proteomic and transcriptomic investigation of fish venom composition using barb tissue from the blue-spotted stingray (Neotrygon kuhlii). Journal of Proteomics. 109C, 188-198. doi: 10.1016/j.prot.2014.06.004. *these authors contributed equally.

Jesupret C, Jackson TNW, Ali SA, Baumann K, Yang D, Greisman L, Kern L, Casewell NR, Undheim EAB, Koludarov I, Debono J, Low DHW, Rossi S, Panagides N, Winters K, Ignjatovic V, Summerhayes R, Jones A, Nouwens A, Dunstan N, Hodgson WC, Winkel K, Monagle P, Fry BG. 2014. Vintage venoms: proteomic and pharmacological stability of snake venoms stored for up to eighty years. Journal of Proteomics. 105, 285-294. doi: 10.1016/j.prot.2014.01.004.

Sunagar K, Casewell NR, Varma S, Kolla R, Antunes A, Moran Y. 2014. Deadly innovations: unraveling the molecular evolution of animal venoms. In: Gopalkrishnakone P (Editor), Calvete JJ (Section Editor), Venom Genomics and Proteomics, Handbooks of Toxinology. Springer Netherlands. doi: 10.1007/978-94-007-6649-5_27-1 

2013

Gutierrez J-M, Warrell DA, Williams DJ, Jensen S, Brown N, Calvete JJ, and Harrison RA. (2013) The need for full integration of snakebite envenoming within a global strategy to combat the Neglected Tropical Diseases: The way forward. PLoS Neglected Tropical Diseases, 7(6) e2162.

Vonk FJ*, Casewell NR*, Henkel CV, Heimburg A, Jansen HJ, McCleary RJR, Kerkkamp HME, Vos R, Guerreiro I, Calvete JJ, Wüster W, Woods AE, Logan JM, Harrison RA, Castoe TA, de Koning APJ, Pollock DD, Renjifo C, Currier RB, Salgado D, Pla D, Sanz L, Hyder AS, Ribeiro JMC, Arntzen JW, van den Thillart GEEJ, Boetzer M, Pirovano W, Dirks RP, Spaink HP, Duboule D, McGlinn E, Kini RM, Richardson MK. 2013. The king cobra genome reveals dynamic evolution and adaptation in the snake venom system. Proc. Natl. Acad. Sci. USA. 110(51), 20651-20656. doi: 10.1073/pnas.1314702110. Cover article. *these authors contributed equally.

Castoe TA, de Koning APJ, Hall KT, Card DC, Schield DR, Fujita MK, Ruggiero RP, Degner JF, Daza JM, Gu W, Reyes-Velasco J, Shaney KJ, Castoe JM, Fox SE, Poole AW, Polanco D, Dobry J, Vandewege MW, Li Q, Schott R, Kapusta A, Minx P, Feschotte C, Uetz P, Ray DA, Hoffman F, Bogden R, Smith EN, Chang BSW, Vonk FJ, Casewell NR, Henkel C, Richardson MK, Mackessy SP, Bronikowski AM, Yandell M, Warren WC, Secor SM, Pollock DD. 2013. The Burmese python genome reveals the molecular basis for extreme adaptation in snakes. Proc. Natl. Acad. Sci. USA. 110(51), 20645-20650. doi: 10.1073/pnas.1314475110.

Casewell NR, Wüster W, Vonk FJ, Harrison RA, Fry BG. 2013. Complex cocktails: the evolutionary novelty of venoms. Trends in Ecology & Evolution. 28, 219-229. doi: 10.1016/j.tree.2012.10.020. Cover article.

Sunagar K, Fry BG, Jackson TNW, Casewell NR, Undheim EAB, Vidal N, Ali SA, King GF, Vasudevan K, Vasconcelos V, Antunes A. 2013. Molecular evolution of vertebrate neurotrophins: co-option of the highly conserved nerve growth factor gene into the advanced snake venom arsenal. PLoS ONE. 8(11), e81827. doi: 10.1371/journal.pone.0081827.

Conlon JM, Attoub S, Arafat H, Mechkarska M, Casewell NR, Harrison RA, Calvete JJ. 2013. Cytotoxic activities of [Ser49] phospholipase A2 from the venom of the saw-scaled vipers Echis ocellatus, E. coloratus, E. pyramidum leakeyi, E. carinatus sochureki. Toxicon 71, 96-104. doi: 10.1016/j.toxicon.2013.05.017.

Brust A, Sunagar K, Undheim EAB, Vetter I, Yang D, Casewell NR, Ruder T, Jackson TNW, Koludarov I, Alewood PF, Hodgson WC, Lewis RJ, King GF, Antunes A, Hendrikx I, Fry BG. 2013. Differential evolution and neofunctionalization of snake venom metalloproteinase domains. Molecular and Cellular Proteomics. 12, 651-663. doi: 10.1074/mcp.m112.023135. 

2012

Fahmi L, Makran B., Pla D, Sanz L. Oukkache N, Lkhider M, Harrison RA, Ghalim N and Calvete JJ. (2012) Venomics and antivenomics profiles of North African Cerastes cerastes and C. vipera populations reveals a potentially important therapeutic weakness. J. Proteomics 75:2442-2453.

Vaiyapuri S, Hutchinson GE, Ali, MS, Dannmoura A, Stanley RG, HarrisonRA, Bicknall AB, and GibbinsJM. (2012) Rhinocetin, a venom–derived integrin-specific antagonist inhibits collagen induced platelet and endothelial cell functions. J Biological Chemistry doi/10.1074/jbc.M112.381483.

Sanz L, Harrison RA, Calvete JJ. (2012) First draft of the genomic organization of a PIII-SVMP gene. Toxicon 60(4):455-69.

Currier RB, Calvete JJ, Sanz L, Harrison RA, Rowley PD, Wagstaff SC (2012) Unusual stability of messenger RNA in snake venom reveals gene expression dynamics of venom replenishment. PLoS One 7(8):e41888.

Casewell NR, Huttley GA, Wüster W. 2012. Dynamic evolution of venom proteins in squamate reptiles. Nature Communications 3, 1066. doi: 10.1038/ncomms2065.

Casewell NR. 2012. On the ancestral recruitment of metalloproteinases into the venom of snakes. Toxicon 60(4), 449-454. doi: 10.1016/j.toxicon.2012.02.006.

Fry BG, Scheib H, Junqueira-de-Azevado ILM, Silva DA, Casewell NR. 2012. Novel transcripts in the maxillary venom glands of advanced snakes. Toxicon 59(7-8), 696-708. doi: 10.1016/j.toxicon.2012.03.005.

Fry BG, Casewell NR, Wüster W, Vidal N, Young B, Jackson T. 2012. The structural and functional diversification of the Toxicoferan reptile venom system. Toxicon 60(4), 434-448. doi: 10.1016/j.toxicon.2012.02.013. 

2011

Vaiyapuri S., Wagstaff, S.C., HarrisonR.A., Watson, K.A., GibbinsJ.M., and Hutchinson G. E. (2011) Evolutionary analysis of novel serine proteases in the venom gland transcriptome of Bitis gabonica rhinoceros. PLoS One 6(6):e21532.

Petras, D., Sanz, L., Segura, A., Herrera, M., Villalta, M., Solano, D., Vargas, M., Leon, G., Warrell, D.A., Theakston, R.D.G., Harrison, R.A., Durfa, N., Nasidi, A., Gutierrez, J-M., Calvete, J.J. (2011) Snake venomics of the African spitting cobras: Toxin composition and assessment of congeneric cross-reactivity of the Pan-African EchiTAb-Plus-ICP antivenom by antivenomics and neutralization approaches. Journal of Proteomic Research in Medecine 10:1266-1280.

Casewell NR, Wagstaff SC, Harrison RA, Renjifo C, Wüster W. 2011. Domain loss facilitates accelerated evolution and neofunctionalization of duplicate snake venom metalloproteinase toxin genes. Molecular Biology and Evolution.  28(9): 2637-2649. doi: 10.1093/molbev/msr091.

Casewell NR, Wagstaff SC, Harrison RA, Wüster W. 2011. Gene tree parsimony of multi-locus snake venom protein families reveals species tree conflict as a result of multiple parallel gene loss. Molecular Biology and Evolution. 28(3): 91-110. doi: 10/1093/molbev/msq302. 

Harrison RA, Cook DA, Renjifo C, Casewell NR, Currier RB, Wagstaff SC. 2011. Research in Medecine strategies to improve snakebite treatment: challenges and progress. Journal of Proteomics. 74(9): 1768-1780. doi: 10.1016/j.jprot.2011.06.019.

Williams DJ, Gutiérrez J-M, Calvete JJ, Wüster W, Ratanabanangkoon K, Paiva O, Brown N, Casewell NR, Harrison RA, Rowley P, Jensen SD, Winkel KD, Warrell DA. 2011. Ending the drought: new strategies for improving the flow of affordable, effective antivenoms in Asia and Africa. Journal of Proteomics. 74(9): 1735-1767. doi: 10.1016/j.jprot.2011.05.027. 

2010

Casewell NR, Cook DAN, Wagstaff SC, Nasidi A, Durfa N, Wüster W, Harrison RA. 2010. Pre-clinical assays predict pan-African Echis viper efficacy for a species-specific antivenom. PLoS Neglected Tropical Diseases. 4(10): e851. doi: 10.1371/journal.pntd.0000851.

Williams D., GutiérrezJ-M., Harrison R.A., WarrellD.A., WhiteJ., WinkelK.D., GopalakrishnakoneP. (2010) The Global Snake Bite Initiative: an antidote for snake bite:  The Lancet, 375, January 2.

Segura A., Villalta M., Herrera M., León G., Harrison R.A., Durfa N., Nasidi A., Calvete  J.J., Theakston R.D.G., Warrell D.A. and Gutiérrez J-M. (2010) Preclinical assessment of the efficacy of a new antivenom (EchiTAb-Plus-ICPÒ) for the treatment of viper envenoming in sub-Saharan Africa. Toxicon, 55:369-374.

Fasoli E., Sanz  L., Wagstaff S., Harrison R.A., Righetti, P.G., Calvete J.J. (2010) Exploring the venom proteome of the African puff adder, Bitis arietans, using a combinatorial library approach at different pHs. Journal of Proteomics 73:932-942.

Currier  R.B., Harrison R.A., Rowley P.D., Laing  G.D. and WagstaffS.C. (2010) Intra-specific variation in venom of the African Puff Adder (Bitis arietans): differential expression and activity of snake venom metalloproteinases (SVMPs). Toxicon, 55:864-873.

Vaiyapuri S., GibbinsJ.M., Harrison R.A., and Hutchinson G. (2010) Purification and functional characterisation of rhinocerase, a novel serine protease from the venom of Bitis gabonica rhinoceros. PLOS One, 5(3):e9687.

Cook, D.A.N., Owen, T., Wagstaff, S.C., Kinne, J., Wernery, U. and Harrison, R.A. (2010a) Analysis of camelid IgG for antivenom development: serological responses of venom-immunised camels to prepare either monospecific or polyspecific antivenoms for West Africa. Toxicon, 56:363-372.

Cook, D.A.N., Owen, T., Wagstaff, S.C., Kinne, J., Wernery, U. and Harrison, R.A. (2010b) Analysis of camelid antibodies for antivenom development: Neutralisation of venom-induced pathology. Toxicon, 56:373-380.

Cook, D.A.N., Samarasekara, C.L., Wagstaff, S.C., Kinne, J., Wernery, U. and Harrison, R.A. (2010c) Analysis of camelid antibodies for antivenom development: Immunoreactivity and preclinical neutralisation of venom-induced pathology by IgG subclasses, and the effect of heat treatment. Toxicon, 56:596-603.

Calvete  J.J., Cid, P., Sanz, L., Segura A., Villalta M., Herrera M., León G., HarrisonR.A., Durfa N., NasidiA., Calvete  J.J., Theakston R.D.G., Warrell D.A. and Gutiérrez J-M. (2010) Antivenomic assessment of the immunological reactivity of EchiTAb-Plus-ICP, an antivenom for the treatment of snakebite envenoming in sub-Saharan Africa. American Journal of Tropical Medicine and Hygiene, 82(6):1194-1201.

Vaiyapuri S., Wagstaff, S.C., Harrison R.A., Watson, K.A., GibbinsJ.M., and Hutchinson G. E. (2010) Purification and Functional Characterization of Rhiminopeptidase A, a Novel Aminopeptidase from the Venom of Bitis gabonica rhinoceros.PLOS Neglected Tropical Diseases 4(8):e796.

Harrison, R.A., Wagstaff, S.C., Cook, D.A.N., Kinne, J., Wernery, U. (2010) Reply to Professor Ratanabanangkoon’s letter entitled ‘D.A.N. Cook et al.’s account of the immunization of camels is at variance with the ‘low dose, low volume multi-site’ immunization protocol. Toxicon 56:1081. 

2009

Casewell NR, Harrison RA, Wüster W, Wagstaff SC. 2009. Comparative venom gland transcriptome surveys of the saw-scaled vipers (Viperidae: Echis) reveal substantial intra-family gene diversity and novel venom transcripts. BMC Genomics.10: 564. doi: 10.1186/1471-2164-10-564.

Wagstaff, S.C., Sanz, L., Juárez, P., Harrison, R.A., and Calvete, J.J. (2009) Combined snake venomics and venom gland transcriptomic analysis of the ocellated carpet viper, Echis ocellatus. Journal of Proteomics, 71:609-623.

Barlow A., Pook C.E., Harrison R.A. and Wüster W. (2009) Coevolution of diet and prey-specific venom activity supports the role of selection in snake venom evolution. Proc. R. Soc. B online pub 2009.0048.

Harrison R.A., Hargreaves A., Wagstaff S.C., Faragher B., and Lalloo D.G. (2009) Snake envenoming: a disease of poverty. PLOS Neglected Tropical Diseases 3 (12):e569

2008

Bazaa, A. Juarez, P., Marrakchi, N., Bel Lasfer, Z., El Ayeb, M., Harrison, R.A. Calvete, J.J. and Sainz, L. (2007) Loss of introns along the evolutionary diversification pathway of snake venom disintegrins evidenced by sequence analysis of genomic DNA from Microvipera lebetina transmediterranea and Echis ocellatus. Journal of Molecular Evolution 64:261-271.

2007

Harrison, R.A. and Wernery, U. (2007) The unique properties of camelid IgG have potential to improve the treatment of snake bite. Journal of Camel Practice and Research in Medecine 14(1):15-16.

Harrison, R.A., Ibison, F., Wilbraham, D. and Wagstaff, SC. (2007) Identification of cDNAs encoding viper venom hyaluronidases: cross-generic sequence conservation of full-length and unusually short variant transcripts. Gene 392:22-32.

Wagstaff, S.C., Favreau, P, Cheneval, O. Laing, G.D., Wilkinson, M.C., Miller, R, Stocklin, R and Harrison R.A. (2008) Molecular characterisation of endogenous snake venom metalloproteinase inhibitors Biochemical and Biophysical Research in Medecine Communications. 365:650-656.

2006

Harrison R.A., Hasson, S.S., Harmsen, M., Laing, G.D., Conrath, K. and Theakston R.D.G.(2006)Neutralisation of venom-induced haemorrhage by IgG from camels and llamas immunised with viper venom and also by endogenous, non-IgG components in camelid sera. Toxicon 47:364-368.

Wagstaff, SC, Laing, G.D., Theakston R.D.G., Papaspyridis, C. and Harrison R.A. (2006) Bioinformatics and multiepitope DNA immunization to design rational snake antivenom PLOS Medicine 3(6):184

Wagstaff, SC and Harrison R.A. (2006) New insights into the mechanisms of Echis ocellatus envenoming by EST sequencing: identification of conserved  integrin binding motifs in SVMPs and molecular characterisation of a new group of putative toxins, the renin-like aspartic proteases. Gene 377:21-32.

Juarez, P., Wagstaff, SC , Sanz, L., Harrison R.A. and Calvete, J. (2006) Molecular cloning of a Bitis arietans disintegrin-like domain, BA-5A, not expressed in the venom proteome. A putative intermediate in the evolution of the long disintegrin bitistatin. Journal of Molecular Evolution 63:142-152.

Juarez, P., Wagstaff, SC , Sanz, L., Harrison R.A. and Calvete, J. (2006) Molecular cloning of Echis ocellatus disintegrins reveals non-expressed messengers and a pathway for the evolution of ocellatusin. Journal of Molecular Evolution 63:183-193